Honduras' public-private partnership promotion agency Coalianza announced that five companies from Honduras, Italy, Asia and the United States are interested in the country's interoceanic rail line.
The freight project is designed to connect the Atlantic and Pacific oceans.
The government has compared the rail line to the Panama Canal and the under-development Nicaragua canal for its potential to expand trade.
The project has a prefeasibility study financed by the Italian government in 2012, including an analysis of possible routes and the potential of Honduran ports Amapala and Puerto Castilla.
The estimated cost of the project is US$10bn and it is expected to be completed by 2030.